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Daniel J. Klionsky, Ph.D.

Professor, Biological Chemistry

A.B., University of California, Los Angeles
Ph.D., Stanford University
Postdoctoral, California Institute of Technology

Research Profile

Like many people, we are interested in understanding the molecular basis of human diseases with an ultimate goal of prevention and/or treatment. Animal cells, however, are difficult to work with experimentally. In contrast, yeast cells provide a relatively simple model system, and studies carried out in yeast are typically applicable to higher eukaryotes. We are interested in the areas of protein targeting and organelle biogenesis. In the past few years, our studies have led us into the area of autophagy. Autophagy is an essential process that allows cell survival under certain stress conditions, yet the molecular details of this process are only now becoming understood. In brief, a cytosolic double membrane vesicle (autophagosome) engulfs portions of cytoplasm. Subsequent fusion with the vacuole (the equivalent of the lysosome) delivers the cargo to the lumen where it can be broken down and recycled. This process plays a normal role in the development of eukaryotic organisms, and is thought to be involved in lifespan extension. On the other hand, autophagic dysfunction is associated with various diseases in humans including cancer and neurodegenerative diseases such as Parkinson's and Alzheimer's disease.

Recent analyses of the autophagy gene products (approximately 27 at present) have begun to provide insight into the molecular mechanism for autophagy and a related biosynthetic process called the cytoplasm to vacuole targeting (Cvt) pathway. For example, both processes require the action of two ubiquitin-like proteins, Atg8 and Atg12, which may act in forming a transient coat that allows formation of the autophagosome. The Cvt pathway may be viewed as a type of specific autophagy. Accordingly, the Cvt pathway, along with other processes including peroxisome degradation, requires components that confer specificity. These proteins, such as Atg11 and Atg19, play roles in cargo recognition and packaging. Continued studies will focus on determining protein interactions, mapping functional domains, reconstituting the sequestration process in vitro and carrying out structural analyses to complete our understanding of this medically relevant process that is fundamental to eukaryotic cell physiology.

Awards

1997 John Simon Guggenheim Memorial Foundation Fellowship
2003 Nat Sci. Foundation Director’s Award for Distinguished Teaching Scholars
2006 Reviewer for John Simon Guggenheim Memorial Foundation
2006 National Academies Education Mentor in the Life Sciences
2009 American Association for the Advancement of Science Fellow

Publications

Abeliovich, H., C. Zhang, W.A. Dunn, Jr., K.M. Shokat and D.J. Klionsky. 2003. Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy. Mol. Biol. Cell, 14: 477-490.

Reggiori, F., C.-W. Wang, P.E. Stromhaug, T. Shintani and D.J. Klionsky. 2003. Vps51 is part of the yeast Vps Fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion.  J. Biol. Chem. 278: 5009-5020.

Tucker, K.A., F. Reggiori, W.A. Dunn Jr. and D.J. Klionsky. 2003. Atg23 is essential for the cytoplasm to vacuole targeting pathway and efficient autophagy but not pexophagy. J. Biol. Chem., 278: 48445-48452.

Wang, C.-W., P.E. Stromhaug, E.J. Kauffman, L.S. Weisman and D.J. Klionsky. 2003. Yeast homotypic vacuole fusion requires the Ccz1-Mon1 complex during the tethering/docking stage. J. Cell Biol., 163: 973-985.

Reggiori, F., K.A. Tucker, P.E. Stromhaug and D.J. Klionsky. 2004. The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure. Dev. Cell, 6: 79-90. PMID: 14723849

Levine, B. and D.J. Klionsky. 2004. Development by Self-Digestion: Molecular mechanisms and biological functions of autophagy. Dev. Cell, 6: 463-477. PMID: 15068787

Reggiori, F., C.-W. Wang, U. Nair, H. Abeliovich and D.J. Klionsky. 2004. Early stages of the secretory pathway, but not endosomes, are required for Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae. Mol. Biol. Cell, 15: 2189-2204. PMID: 15004240

Budovskaya, Y.V., J.S. Stephan, F. Reggiori, D.J. Klionsky and P.K. Herman. 2004. The Ras/PKA signaling pathway regulates an early step of the autophagy process in Saccharomyces cerevisiae. J. Biol. Chem. 279: 20663-20671

Shintani, T. and D.J. Klionsky. 2004. Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway. J. Biol. Chem., 279: 29889-29894. PMID: 15138258

Strømhaug, P.E., F. Reggiori, J. Guan, C.-W. Wang and D.J. Klionsky. 2004. Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy. Mol. Biol. Cell, in press. PMID: 15155809

Klionsky, D.J. 2004 Regulated self-cannibalism. Nature, 431: 31-32.

Shintani, T., and D.J. Klionsky. 2004. Autophagy in health and disease: a double-edged sword. Science, 306: 990-995.

Klionsky, D.J. 2004. Talking biology: Teaching outside the textbook, and the lecture. Cell Biol. Ed., 3: 204-211.

Klionsky, D.J. 2005. The molecular machinery of autophagy: Unanswered questions. J. Cell Sci., 118: 7-18.

Yorimitsu, T., and D.J. Klionsky. 2005. Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol. Biol. Cell, 16: 1593-1605.

Nazarko, T.Y., J. Huang, J.-M. Nicaud, D.J. Klionsky, and A.A. Sibirny. 2005. Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy, 1: 37-45.

Klionsky, D.J., A.J. Meijer, P. Codogno, T.P. Neufeld, and R.C. Scott. 2005. Autophagy and p70S6 kinase. Autophagy, 1: 59-60.

Klionsky, D.J. 2005. Autophagy. Current Biology, 15: R282-283.

Reggiori, F., and D.J. Klionsky. 2005. Autophagosomes: biogenesis from scratch? Curr. Opin. Cell Biol., 17: 415-422.

Reggiori, F., T. Shintani, U. Nair, and D.J. Klionsky. 2005. Atg9 cycles between mitochondria and the pre-autophagosomal structure in yeasts. Autophagy, 1: 101-109.

Cheong, H., T. Yorimitsu, F. Reggiori, J.E. Legakis, C.-W. Wang, and D.J. Klionsky. 2005. Atg17 regulates the magnitude of the autophagic response. Mol. Biol. Cell, 16: 3438-3453.

Towns, R., Y. Kabeya, T. Yoshimori, C. Guo, Y. Shangguan, S. Hong, M. Kaplan, D.J. Klionsky, and J.W. Wiley. 2005. Sera from patients with type 2 diabetes and neuropathy induce autophagy and colocalization with mitochondria in SY5Y cells. Autophagy 1: 163-170.

Yorimitsu, T., and D.J. Klionsky. 2005. Autophagy: molecular machinery for self-eating. Cell Death Diff., 12: 1542-1552.

Nair, U. and D.J. Klionsky. 2005. Molecular mechanisms and regulation of specific and nonspecific autophagy pathways in yeast. J. Biol. Chem., 280: 41785 - 41788. [Cover illustration]

Reggiori, F., I. Monastyrska, T. Shintani and D.J. Klionsky. 2006. The actin cytoskeleton is essential for selective autophagy in the yeast Saccharomyces cerevisiae. Mol. Biol. Cell, 16: 5843-5856.

Klionsky, D.J. and A. Kumar. 2006. A systems biology approach to learning autophagy. Autophagy, 2: 12-23.

Monastyrska, I., T. Shintani, D.J. Klionsky, and F. Reggiori. Atg11 directs autophagosome cargoes to the PAS along actin cables. Autophagy 2: 119-121.

Klionsky, D.J. 2006. Why don't we teach the telephone book? Teach. Prof. 20:7.

He, C. and D.J. Klionsky. 2006. Autophagy and neurodegeneration. ACS Chem. Biol., 1: 211-213.

Klionsky, D.J. 2006. Good riddance to bad rubbish. Nature, 441: 819-820.

Legakis, J.E. and D.J. Klionsky. 2006. Overview of autophagy. In Autophagy in Immunity and Infection. A Novel Immune Effector (Deretic, V. ed). Wiley-VCH, Weinheim, pp. 3-17.

Reggiori, F. and D.J. Klionsky. 2006. Atg9 sorting from mitochondria is impaired in early secretion and VFT complex mutants in Saccharomyces cerevisiae. J. Cell Sci., 119: 2903-2911.

Monastyrska, I. and D.J. Klionsky. 2006. Autophagy in organelle homeostasis: Peroxisome turnover. Mol. Aspects Med., 27: 483-494.

Yorimitsu, T., U. Nair, Z. Yang and D.J. Klionsky. 2006. Endoplasmic reticulum stress triggers autophagy. J. Biol. Chem., 281: 30299-30304.

Yang, Z., J. Huang, J. Geng, U. Nair and D.J. Klionsky. 2006. Atg22 recycles amino acids to link the degradative and recycling functions of autophagy. Mol. Biol. Cell, 17: 5094-5104.

He, C., H. Song, T. Yorimitsu, I. Monastyrska, W.-L. Yen, J.E. Legakis and D.J. Klionsky. 2006. Recruitment of Atg9 to the pre-autophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. J. Cell Biol., 175: 925-935.

Cao, Y. and D.J. Klionsky. 2007. Atg26 is not involved in autophagy-related pathways in Saccharomyces cerevisiae. Autophagy, 3: 17-20.

Yen, W.-L., J.E. Legakis, U. Nair and D.J. Klionsky. 2007. Atg27 is required for autophagy-dependent cycling of Atg9. Mol. Biol. Cell. 18: 581-593.

Klionsky, D.J. 2007. Monitoring autophagy in yeast: The Pho8∆60 assay. In Methods in Molecular Biology: Protein Targeting Protocols, 2nd ed. (M. van der Giezen, ed.) The Humana Press Inc., Totowa, NJ. 390: 363-371.

Tal, R., G. Winter, N. Ecker, D.J. Klionsky and H. Abeliovich. 2007. Aup1p, a yeast mitochondrial protein phosphatase homolog, is required for efficient stationary phase mitophagy and cell survival. J. Biol. Chem., 282: 5617-5624.

Klionsky, D.J. 2007. Malpractice insurance for university professors? Teach. Prof, 21: 6.

Yang, Z. and D.J. Klionsky. 2007. Permeases recycle amino acids resulting from autophagy. Autophagy, 3: 149-150.

Yorimitsu, T. and D.J. Klionsky. 2007. Endoplasmic reticulum stress: a new pathway to induce autophagy. Autophagy, 3: 160-162.

Mizushima, N. and D.J. Klionsky. 2007. Protein turnover via autophagy: implications for metabolism. Annu. Rev. Nutr., 27: 19-40.

Legakis, J.E., W.-L. Yen and D.J. Klionsky. 2007. A cycling protein complex required for selective autophagy. Autophagy, 3: 422-432.

Yen, W-L. and D.J. Klionsky. 2007. Atg27 is a second transmembrane cycling protein. Autophagy, 3: 254-256.

He, C. and D.J. Klionsky. 2007. Atg9 trafficking in autophagy-related pathways. Autophagy, 3: 271-274.

Klionsky, D.J., A.M. Cuervo, and P.O. Seglen. 2007. Methods for monitoring autophagy from yeast to human. Autophagy, 3: 181-206.

Rubinsztein, D.C., J.E. Gestwicki, L.O. Murphy and D.J. Klionsky. 2007. Potential therapeutic applications of autophagy. Nat. Rev. Drug Disc., 6: 304-312.

Huang, J., F. Reggiori and D.J. Klionsky. 2007. The transmembrane domain of acid trehalase mediates ubiquitin-independent Multivesicular body pathway sorting. Mol. Biol. Cell, 18: 2511-2524.

Yorimitsu, T. and D.J. Klionsky. 2007. Eating the endoplasmic reticulum: Quality control by autophagy., Trends Cell Biol., 17:279-285.

Gramatikoff, K., A. Erishkin, J. Montzouris, N. Mizushima, M. Hochstrasser, J.W. Smith, A.M. Cuervo, D.J. Klionsky. Macroautophagy (poster)

Mijaljica, D., M. Prescott, D.J. Klionsky and R.J. Devenish. 2007. Autophagy and vacuole homeostasis: a case for self-degradation? Autophagy, 3: 417-421.

Huang, J. and D.J. Klionsky. 2007. Autophagy and Human Disease. Cell Cycle, 6: 1837-1849.

Yorimitsu, T., S. Zaman, J.R. Broach and D.J. Klionsky. 2007. Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae. Mol. Bio. Cell, 18: 4180-4189.

Ma, J., R. Jin, X. Jia, C.J. Dobry, L. Wang, F. Reggiori, J. Zhu, and A. 2007, Kumar An interrelationship between autophagy and filamentous growth in budding yeast. Genetics, 177: 205-214.

Xie, Z. and D.J. Klionsky. 2007. Autophagosome formation: core machinery and adaptations. Nat. Cell Biol., 9: 1102-1109.

Cao, Y. and D.J. Klionsky. 2007. Physiological functions of Atg6/Beclin 1: a unique autophagy-related protein. Cell Res. 17: 839-849.

Klionsky, D.J. 2007. Autophagy: from phenomenology to molecular understanding in less than a decade. Nat. Rev. Mol. Cell Biol., 8: 931-937.

Klionsky, D.J. 2007. From the Future: A Brief History of Pedagogy. Teaching Prof., 21: 5.

Cheong, H., U. Nair, J. Geng and D.J. Klionsky. 2008. The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae. Mol. Biol. Cell, 19: 668-681.

Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, Bamber BA, Bassham DC, Bergamini E, Bi X, Biard-Piechaczyk M, Blum JS, Bredesen DE, Brodsky JL, Brumell JH, Brunk UT, Bursch W, Camougrand N, Cebollero E, Cecconi F, Chen Y, Chin L-S, Choi A, Chu CT, Chung J, Clark RSB, Clarke PGH, Clarke SG, Clave C, Cleveland JL, Codogno P, Colombo MI, Coto-Montes A, Cregg JM, Cuervo AM, Debnath J, Dennis PB, Dennis PA, Demarchi F, Deretic V, Devenish RJ, Di Sano F, Dice JF, Distelhorst CW, Dinesh-Kumar S, DiFiglia M, Djavaheri-Mergny M, Dorsey FC, Dröge W, Dron M, Dunn WAJ, Duszenko M, Eissa NT, Elazar Z, Esclatine A, Eskelinen E-L, Fésüs L, Finley K, Fuentes JM, Fueyo J, Fujisaki K, Galliot B, Gao F-B, Gewirtz DA, Gibson SB, Gohla A, Goldberg AL, Gonzalez R, González-Estévez C, Gorski S, Gottlieb RA, Häussinger D, He Y-W, Heidenreich K, Hill JA, Høyer-Hansen M, Hu X, Huang W-P, Iwasaki A, Jäättelä M, Jackson WT, Jiang X, Jin S, Johansen T, Jung JU, Kadowaki M, Kang C, Kelekar A, Kessel DH, Kiel JAKW, Kim HP, Kimchi A, Kinsella TJ, Kiselyov K, Kitamoto K, Knecht E, Komatsu M, Kominami E, Kondo S, Kovács AL, Kroemer G, Kuan C-Y, Kumar R, Kundu M, Landry J, Laporte M, Le W, Lei H-Y, Levine B, Lieberman A, Lim K-L, Lin F-C, Liou W, Liu LF, Lopez-Berestein G, López-Otín C, Lu B, Macleod KF, Malorni W, Martinet W, Matsuoka K, Mautner J, Meijer AJ, Meléndez A, Michels P, Miotto G, Mistiaen WP, Mizushima N, Mograbi B, Moore MN, Moreira PI, Moriyasu Y, Motyl T, Münz C, Murphy LO, Naqvi NI, Neufeld TP, Nishino I, Nixon RA, Noda T, Nürnberg B, Ogawa M, Oleinick NL, Olsen LJ, Ozpolat B, Paglin S, Palmer GE, Papassideri IS, Parkes M, Perlmutter DH, Perry G, Piacentini M, Pinkas-Kramarski R, Prescott M, Proikas-Cezanne T, Raben N, Rami A, Reggiori F, Rohrer B, Rubinsztein DC, Ryan KM, Sadoshima J, Sakagami H, Sakai Y, Sandri M, Sasakawa C, Sass M, Schneider C, Seglen PO, Seleverstov O, Settleman J, Shacka JJ, Shapiro IM, Sibirny A, Silva-Zacarin ECM, Simon H-U, Simone C, Simonsen A, Smith MA, Spanel-Borowski K, Srinivas V, Steeves M, Stenmark H, Stromhaug PE, Subauste CS, Sugimoto S, Sulzer D, Suzuki T, Swanson MS, Tabas I, Takeshita F, Talbot NJ, Tallóczy Z, Tanaka K, Tanaka K, Tanida I, Taylor GS, Taylor JP, Terman A, Tettamanti G, Thompson CB, Thumm M, Tolkovsky AM, Tooze SA, Truant R, Tumanovska LV, Uchiyama Y, Ueno T, Uzcátegui NL, van der Klei IJ, Vaquero EC, Vellai T, Vogel MW, Wang H-G, Webster P, Xi Z, Xiao G, Yahalom J, Yang J-M, Yap GS, Yin X-M, Yoshimori T, Yue Z, Yuzaki M, Zabirnyk O, Zheng X, Zhu X, Deter RL. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 2008; 4: 151-175.

Legakis, J.E. and D.J. Klionsky. 2008. Analysis of autophagosome membrane cycling by fluorescence microscopy. In Autophagosome and Phagosome (Deretic, V. ed). Humana, 135-145.

Mizushima, N., B. Levine, A.M. Cuervo and D.J. Klionsky. 2008. Autophagy fights disease through cellular self-digestion. Nature, 451: 1069-1075.

Monastyrska, I., C. He, J. Geng, A.D. Hoppe, Z. Li and D.J. Klionsky. 2008. The actin-related protein Arp2 is required for Atg9 movement during autophagy. Mol. Biol. Cell, 19: 1962-1975.

Deretic, V. and D.J. Klionsky. 2008. How cells clean house. Sci. American, 298: 74-81.

Geng, J., M. Baba, U. Nair and D.J. Klionsky. 2008. Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy. J. Cell Biol., 182: 129-140.

Xie, Z., U. Nair and D.J. Klionsky. 2008. Atg8 controls phagophore expansion during autophagosome formation. Mol. Biol. Cell, 19: 3290-3298.

Cao, Y., H. Cheong, H. Song and D.J. Klionsky. 2008. In vivo reconstitution of autophagy in Saccharomyces cerevisiae. J. Cell Biol., 182: 703-713.

Klionsky, D.J. 2008. The quiz factor. Cell Biol. Ed., 7: 265-266.

Cheong, H. and D.J. Klionsky. 2008. Dual role of Atg1 in regulation of autophagy-specific PAS assembly in Saccharomyces cerevisiae. Autophagy 4: 724-726.

Klionsky, D.J. 2008. Autophagy revisited: a conversation with Christian de Duve. Autophagy 4: 740-743.

Monastyrska, I., F. Reggiori and D.J. Klionsky. 2008. Harpooning the Cvt complex to the phagophore assembly site. Autophagy 4: 914-916.

Xie, Z., U. Nair and D.J. Klionsky. 2008. Dissecting autophagosome formation: the missing pieces. Autophagy 4: 920-922.

Geng, J. and D.J. Klionsky. 2008. Quantitative regulation of vesicle formation in yeast non-specific autophagy. Autophagy 4: 955-957.

Geng, J. and D.J. Klionsky. 2008. The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. EMBO Rep. 9: 859-864.

Yen, W.-L. and D.J. Klionsky. 2008. How to Live Long and Prosper: Autophagy, Mitochondria, and Aging. Physiol., 23: 248-262.

Cao, Y. and D.J. Klionsky. 2008. New insights into autophagy using a multiple knockout strain. Autophagy 4: 1073-1075.

He, C., M. Baba, Y. Cao and D.J. Klionsky. 2008. Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy. Mol. Biol. Cell 19: 5506-5516.

Kanki, T. and D.J. Klionsky. 2008. Mitophagy in yeast occurs through a selective mechanism. J. Biol. Chem. 283: 32386-32393.

Klionsky, D.J. and R.L. Deter. 2008. In the autophagy trenches: The early research career of Russell L. Deter. Autophagy 4: 978-981.

Cheong, H. and D.J. Klionsky. 2008. In Autophagy: Lower eukaryotes and non-mammalian systems, Part A (ed. D.J. Klionsky). Analysis of the cytoplasm to vacuole targeting pathway and autophagy. Methods Enzymol. Vol. 451: 1-26. Academic Press.

Noda, T. and D.J. Klionsky. 2008. In Autophagy: Lower eukaryotes and non-mammalian systems, Part A (ed. D.J. Klionsky). The quantitative Pho8∆60 assay of non-specific autophagy. Methods Enzymol. Vol. 451: 33-42.

Klionsky, D.J. (ed) 2008. Autophagy: Lower Eukaryotes and non-Mammalian Systems-Part A. Methods Enzymol. 451.

Xie, Z., U. Nair, J. Geng, M.B. Szefler, E.D. Rothman and D.J. Klionsky. 2009. Indirect estimation of the area density of Atg8 on the phagophore. Autophagy 5: 217-220.

Klionsky, D.J. (ed) 2009. Autophagy: Mammals and Clinical-Part B. Methods Enzymol. 452.

Klionsky, D.J. (ed) 2009. Autophagy: Mammals and Clinical-Part C. Methods Enzymol. 453.

Sirum, K.L., D. Madigan and D.J. Klionsky. 2009. Enabling a culture of change: A life sciences faculty learning community promotes scientific teaching. J. Coll. Sci. Teach., 38: 24-30.

Klionsky, D.J. 2009. Crohn's disease, autophagy, and the Paneth cell. New Engl. J. Med. 360: 1785-1786.

Monastyrska, I., D.J. Klionsky and F. Reggiori. 2009. Multiple roles of the cytoskeleton in autophagy. Biol. Rev., 84: 431-448.

He, C., M. Baba and D.J. Klionsky. 2009. Double duty of Atg9 self-association in autophagosome biogenesis. Autophagy 5: 385-387.

He, C., C. Bartholomew, W. Zhou and D.J. Klionsky. 2009. Assaying autophagic activity in transgenic GFP-Lc3 and GFP-gabarap zebrafish embryos. Autophagy 5: 520-526.

Yorimitsu, T., C. He, K. Wang and D.J. Klionsky. 2009. Tap42-associated protein phosphatase type 2A negatively regulates induction of autophagy. Autophagy 5: 616-624.

Cao, Y., U. Nair, K. Yasumura-Yorimitsu and D.J. Klionsky. 2009. A multiple ATG gene knockout strain for yeast two-hybrid analysis. Autophagy 5: 699-705.

Kanki, T., K. Wang, Y. Cao, M. Baba and D.J. Klionsky. 2009. Atg32 is a mitochondrial protein that confers selectivity during mitophagy. Dev. Cell, 17: 98-109.

Kanki, T. and D.J. Klionsky. 2009. Mitochondrial abnormalities drive cell death in Wolfram syndrome 2. Cell Res., 19: 922-923.

He, C. and D.J. Klionsky. 2009. Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genetics 43: 67-93.

Galluzzi, L., S.A. Aaronson, J. Abrams, E.S. Alnemri, D.W. Andrews, E.H. Baehrecke, N.G. Bazan, M.V. Blagosklonny, K. Blomgren, C. Borner, D.E. Bredesen, C. Brenner, M. Castedo, J.A. Cidlowski, A. Ciechanover, G.M. Cohen, V. De Laurenzi, R. De Maria, M. Deshmukh, B.D. Dynlacht, W.S. El-Deiry, R.A. Flavell, S. Fulda, C. Garrido, P. Golstein, M.-L. Gougeon, D.R. Green, H. Gronemeyer, G. Hajnóczky, J.M. Hardwick, M.O. Hengartner, H. Ichijo, M. Jääattelä, O. Kepp, A. Kimchi, D.J. Klionsky, R.A. Knight, S. Kornbluth, S. Kumar, B. Levine, S.A. Lipton, E. Lugli, F. Madeo, W. Malorni, J.-C.W. Marine, S.J. Martin, J.P. Medema, P. Mehlen, G. Melino, U.M. Moll, E. Morselli, S. Nagata, D.W. Nicholson, P. Nicotera, G Nuñez, M. Oren, J. Penninger, S. Pervaiz, M.E. Peter, M. Piacentini, J.H.M. Prehn, H. Puthalakath, G.A. Rabinovich, R. Rizzuto, C.M.P. Rodrigues, D.C. Rubinsztein, T. Rudel, L. Scorrano, H.-U. Simon, H. Steller, J. Tschopp, Y. Tsujimoto, P. Vandenabeele, I. Vitale, K.H. Vousden, R.J. Youle, J. Yuan, B. Zhivotovsky and G. Kroemer2009. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. Cell Death Differ. 16: 1093-1107.

Klionsky, D.J. 2009. Putting the upper division cart before the introductory horse. Cell Biol. Ed., 8: 155-156.

Vellai, T., K. Takács-Vellai, M. Sass and D.J. Klionsky. The regulation of aging: does autophagy underlie longevity? Trends Cell Biol., 19: 487-494.

Yang, Z. and D.J. Klionsky. 2009. An overview of the molecular mechanism of autophagy. Curr. Top. Microbiol. Immunol. 335: 1-32.

Klionsky, D.J. and J.F. Dice. 2009. Chaperone-mediated autophagy: The heretofore untold story of J. Fred "Paulo" Dice. Autophagy 5: 1079-1084.

Kanki, T. and D.J. Klionsky. 2009. Atg32 is a tag for mitochondria degradation in yeast. Autophagy 5: 1201-1202.

Kanki, T., K. Wang, M. Baba, C.R. Bartholomew, M.A. Lynch-Day, Z. Du, J. Geng, K. Mao, Z. Yang, W.-L. Yen and D.J. Klionsky. 2009. A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol. Biol. Cell, 20: 4730-4738.

Kanki, T., D. Kang and D.J. Klionsky. 2009. Monitoring mitophagy in yeast: The Om45-GFP processing assay. Autophagy 5: 1186-1189.

Klionsky, D.J. 2009. A report from the EMBO conference on autophagy. Autophagy 5: 1235-1239.

Brest, P., E.A. Corcelle, A. Cesaro, A. Chargui, A. Belaïd, D.J. Klionsky, V. Vouret-Craviari, X. Hebuterne, P. Hofman and B. Mograbi. 2009. AUTOPHAGY AND CROHN'S DISEASE: at the crossroads of infection, inflammation, immunity, and cancer. Curr. Mol. Med., in press.

Yen, W.-L., T. Shintani, U. Nair, Y. Cao, B.C. Richardson, Z. Li, F.M. Hughson, M. Baba and D.J. Klionsky. 2009. The Conserved Oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy. J. Cell Biol., in press.

Yang, Z. and D.J. Klionsky. 2009. Mammalian autophagy: core molecular machinery and signaling regulation. Curr. Opin. Cell Biol., in press.

klionsky@umich.edu

Office: 6036 LSI, Box 2216
PH: (734)615-6556

PubMed: klionsky dj

Department Affiliations

Life Sciences Institute